Mass production of Siberian Ginseng (Eleutherococcus senticosus) somaticembryos by cell culturing > Volume 01 - 2001

본문 바로가기

사이트 내 전체검색


 » New Articles(2011~Present)
 » Articles (2001~2010)
      ∙ Volume 10 - 2010
      ∙ Volume 09 - 2009
      ∙ Volume 08 - 2008
      ∙ Volume 07 - 2007
      ∙ Volume 06 - 2006
      ∙ Volume 05 - 2005
      ∙ Volume 04 - 2004
      ∙ Volume 03 - 2003
      ∙ Volume 02 - 2002
      ∙ Volume 01 - 2001
 

Oriental Pharmacy and Experimental Medicine
Volume 01 - 2001
Date: 2001

Journal: Pages 34-38

August 2001 | Mass production of Siberian Ginseng (Eleutherococcus senticosus) somaticembryos by cell culturing…

Jae-Whune Kim1* and Ha-Song Kim2

첨부파일

Summary

​Somatic embryogenic cells of Siberian ginseng (Eleutherococcus senticosus or Acanthopanax senticosus)were cultured in MS liquid medium by subculture at 2 week intervals. The embryogenic cellswere filtered through a 250 mm sieve and the passed cells could proliferate with maintenance ofsomatic embryos. The embryogenic cell clusters entrapped on the sieve were transferred to an MSmedium without growth regulators and were cultured under fluorescent light in the half shade.The cell clumps developed to somatic embryos of uniform sizes of torpedo stage after 4~5 weeksof culture. The somatic embryos in a torpedo stage were found to gain in fresh weight about 20times greater after culturing in the bio-reactors. The somatic embryos in a torpedo stage weretransferred to 510 L air-left type bioreactors. The culturing for 10~15 days led the somaticembryos to the development of seedlings which could be utilized as materials for health foods orproviding useful components.


This website Copyright (c) 2014 Institute of Korean Medicine Kyung Hee University All rights reserved.
Top View
Mobile View