Euphorbia hirta, locally called ‘ara tanah’ or ‘susun nabi’ in Malaysia is a small annual herbcommon to the tropical countries and belongs to the same family as the tic and tapioca. E. hirtahas had a long history of usage in the treatment of various ailments. In this current study, in vitrosensitivity test of crude aqueous and ethanol extracts of leaves and barks of E. hirta was carriedout against bacteria (Escherichia coli, Salmonella enteritidis, Staphylocccus aureus and Bacillus subtilis)and fungi (Microsporum canis, Aspergillus fumigatus, Candida albicans and Candida tropicalis) usingthe discs diffusion method. The extract-impregnated discs (20, 40 and 80 mg/ml), the E. hirtaextracts inhibited the growth of all the bacteria tested. The growth of C. albicans was inhibited in aconcentration dependent manner by the aqueous leaves and barks extracts. C. tropicalis was foundto be sensitive to the aqueous leaves extracts. The results were compared to antibacterial drugs ofchloramphenicol, ampicilin, penicillin G, and enrofloxacine; and to antifungal drug ofketoconazole, itraconazole and miconazole. In this current study, it can be concluded that thisplant has antimicrobial activity that is as potent as the standard antimicrobial drugs againstcertain microorganisms.