The purpose of this study was to examine the effect of supplementary highly purified chitosan(HPC) on blood alcohol concentration in healthy human. The human study was performed withtwo sections. Each section of the study was conducted by two-phase cross-over design with aweek wash-out period. All volunteers took HPC in one phase, and took a placebo in the nextphase. Blood alcohol concentrations were different between in those taking HPC and in thosetaking the placebo in the human. And the concentration of serum aspartate aminotransferase(AST, GOT) and alanine aminotransferase (ALT, GPT), the indicator of liver cell damage, waslowered in those taking HPC, compared to those taking the placebo. In conclusion, taking HPCprior to drinking alcohol can somewhat reduce alcohol concentration in human blood and livercell damage.