We studied the inhibitory effect of Green Life Enzyme (GLE) on compound 48/80-induced anaphylacticresponse in a murine model. GLE inhibited compound 48/80-induced systemic anaphylacticshock at the dose of 10g/kg by 87.5%. When GLE was given as pre-treatment at concentrationsranging from 0.01 to 1.0g/kg, it inhibited passive cutaneous anaphylaxis activated by anti-dinitrophenyl (DNP) IgE. In addition, GLE (0.1mg/ml) inhibited anti-DNP IgE-induced tumor necrosisfactor-a production from mast cells by 69% compared to saline value. These results indicate that GLEmay possess anti-anaphylactic and anti-inflammatory activity.