Stephania hernandifolia belonging to the family Menispermaceae is the biggest storehouse ofdiphenylbisbenzylisoquinoline (DBBI) alkaloids. Exhaustive chemical processing of the bulb of S.hernandifolia by the application of modern separation techniques yielded a DBBI alkaloid whichwas identified as cycleanine using spectroscopic methods (UV, IR, 1HNMR. 13CNMR, Mass). Cycleanineshowed significant anti-inflammatory activity against carrageenin induced paw oedema,comparable to that produced by diclofenac sodium, used as standard drug. It exhibited potentanalgesic effects against chemical and thermal noxious stimuli. It was also found to possess anti-convulsive activity in the strychnine induced convulsion model.