Polysaccharide hydrogels constitute a structurally diverse class of biological macromoleculeswith a wide range of physicochemical properties. They also constitute important members of thefamily of industrial water-soluble polymers. They find application in Pharmacy as binders,disintegrants, suspending, emulsifying and sustaining agents. According to the InternationalPharmaceutical Excipients Council (IPEC), an excipient must have an established safety profile.Hence, in the present study, in vitro cytotoxicity on Vero and HEp-2 cell lines, and in vivo acutetoxicity in rats were carried out to establish the safety of polysaccharide hydrogels from the seedsof Plantago ovata and Ocimum basilicum. The in vitro cytotoxicity was determined by MTT and SRBassays. In the in vivo acute toxicity, the effects of three different doses of hydrogels (100, 200 and400 mg/kg body weight) on food and water intake, body weight, biochemical and hematologicalparameters were studied. The results of in vitro did not show any cytotoxicity on both the celllines used. In the in vivo acute toxicity, the hydrogels did not show any toxic symptoms in allthree dose levels. This establishes the safety of the selected hydrogels. Hence, they can be used asexcipients in pharmaceutical dosage forms.