In vitro cytotoxicity and in vivo acute toxicity of selected polysaccharidehydrogels as pharmaceutical excipients > Volume 05 - 2005

본문 바로가기

사이트 내 전체검색


 » New Articles(2011~Present)
 » Articles (2001~2010)
      ∙ Volume 10 - 2010
      ∙ Volume 09 - 2009
      ∙ Volume 08 - 2008
      ∙ Volume 07 - 2007
      ∙ Volume 06 - 2006
      ∙ Volume 05 - 2005
      ∙ Volume 04 - 2004
      ∙ Volume 03 - 2003
      ∙ Volume 02 - 2002
      ∙ Volume 01 - 2001
 

Oriental Pharmacy and Experimental Medicine
Volume 05 - 2005
Date: 2005

Journal: Pages 29-36

March 2005 | In vitro cytotoxicity and in vivo acute toxicity of selected polysaccharidehydrogels as pharmaceutical excipients…

Kulkarni GT*, Gowthamarajan K, Raghu C, Ashok G and Vijayan P

첨부파일

Summary

​Polysaccharide hydrogels constitute a structurally diverse class of biological macromoleculeswith a wide range of physicochemical properties. They also constitute important members of thefamily of industrial water-soluble polymers. They find application in Pharmacy as binders,disintegrants, suspending, emulsifying and sustaining agents. According to the InternationalPharmaceutical Excipients Council (IPEC), an excipient must have an established safety profile.Hence, in the present study, in vitro cytotoxicity on Vero and HEp-2 cell lines, and in vivo acutetoxicity in rats were carried out to establish the safety of polysaccharide hydrogels from the seedsof Plantago ovata and Ocimum basilicum. The in vitro cytotoxicity was determined by MTT and SRBassays. In the in vivo acute toxicity, the effects of three different doses of hydrogels (100, 200 and400 mg/kg body weight) on food and water intake, body weight, biochemical and hematologicalparameters were studied. The results of in vitro did not show any cytotoxicity on both the celllines used. In the in vivo acute toxicity, the hydrogels did not show any toxic symptoms in allthree dose levels. This establishes the safety of the selected hydrogels. Hence, they can be used asexcipients in pharmaceutical dosage forms.


This website Copyright (c) 2014 Institute of Korean Medicine Kyung Hee University All rights reserved.
Top View
Mobile View