“SecSec” has been used for the purpose of prevention and treatment of throat diseases such assore throat, cough, bronchial asthma and allergic asthma in Korea. However, its effect inexperimental models remains unknown. To investigate the biological effect of SecSec, weexamined cytotoxicity and secretion of inflammatory cytokines on human leukemic mast cell line,HMC-1, stimulated with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187.SecSec by itself had no cytotoxicity on HMC-1. When SecSec (1 mg/ml) was added, the secretionof tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and granulocyte macrophage-colonystimulating factor (GM-CSF) was significantly inhibited about 47.20%, 25.55%, and 46.43%,respectively on PMA plus A23187-stimulated HMC-1 cells. But SecSec did not inhibit IL-8secretion. These findings may help understanding the mechanism of action of this medicineleading to control activated mast cells on allergic inflammatory condition like asthma.