The Angelicae dahuricae radix (ADR) has been used a traditional medicine to treat acne, erythema,headache, toothache, sinusitis, colds, and flu in Korea, Japan and China. Here, we report the effectof ADR on compound 48/80-induced ear-swelling and the phorbol myristate acetate (PMA) pluscalcium ionophore A23187-induced inflammatory cytokine secretion in the human mast cell line,HMC-1. ADR dose-dependently inhibited the ear-swelling response induced by intradermalinjection of compound 48/80. In vitro model, PMA plus A23187 significantly increasedinterleukin (IL)-1β, IL-8, granulocyte macrophage colony stimulating factor (GM-CSF), and tumornecrosis factor (TNF)-α secretion compared with media control. We also show that the increasedcytokines IL-1β, IL-8, GM-CSF, and TNF-α level was significantly inhibited by treatment of ADR.In addition, ADR partially blocked PMA plus A23187-induced extracelluar signal-regulatedkinases phosphorylation. These results suggest that ADR might explain its beneficial effect in thetreatment of mast cell-mediated inflammatory diseases.