This study was conducted to investigate the efficacy of antioxidative activity and nitritescavenging ability of each fractions from Phyllostachys bambusoides S. et Z. (P. bambusoides) trunkethanolic extract using reverse-phase flash chromatography. Among the each fractions, fraction 3(H2O:MeOH = 1:1) showed high DPPH free radical scavenging activity (81.33%) at 80 µg/mLconcentrations and strongly inhibited autooxidation of pyrogallol by superoxide dismutase-likeactivity (45.8%) at 0.46 mg/mL concentrations compared with different fractions. The fraction 3was also increased to 76.62% cell viability against hydrogen peroxide-mediated cytotoxicity.Nitrite scavenging ability was the most remarkable under pH 1.2 condition among various pHregions examined and effectively exhibited to 65.6% by treatment of the fraction 3 with aconcentration of 0.2 mg/ml. In general, nitrite scavenging ability decreased with higher pHcondition. These results suggest that fraction 3 from P. bambusoides ethanolic extract can be usedfor bioacitve and functional materials.