A number of species of the genus Centaurea (Family: Asteraceae), distributed in various parts ofAsia, Europe and North America, have been used in traditional plant-based medicine andreported to possess various medicinal properties. As part of our continuing evaluation of plantsfrom the genus Centaurea for their phytochemistry and biological activities, the dichloromethane(DCM) and methanol (MeOH) extracts of the seeds of Turkish Centaurea species, C. bornmuelleri,C. huber-morathii and C. schiskinii, were screened for antioxidant and antibacterial activities.Among the three species, C. huber-morathii displayed the most prominent antibacterial activity.Both the MeOH and DCM extracts of this plant showed activity against Citrobacter freundii,Enterococcus faecalis and Salmonella goldcoast with the MIC values within the range of 1 × 10-2 to 1 ×10-3 mg/ml. The MeOH extract of C schiskinii showed activity (MIC = 1 × 10-1 mg/ml) againstCitrobacter freundii and Staphylococcus aureus. While the DCM extract of C. bornmuelleri was onlyactive against Staphylococcus aureus (MIC = 1 × 10-2 mg/ml), the MeOH extract did not show anyinhibitory activity at test concentrations. The DCM and MeOH extracts of all three speciesdemonstrated good degree of antioxidant property in the DPPH assay with the RC50 valuesranging from 72 × 10-2 to 31 × 10-3 mg/ml. Among these extracts, the MeOH extract of C. huber-morathii was the most active antioxidant extract (MIC = 31 × 10-3 mg/ml).