The plant Bryonia laciniosa (Family: Cucurbitaceae) has been indicated for the treatment of variousdiseases one among which is cancer. The purpose of this study was investigating experimentally thepossible anti-tumor effect and antioxidant role of Bryonia laciniosa leaves in animal model. Themethanol extract of Bryonia laciniosa (MEBL) administered at the doses of 62.5, 125 and 250 mg/kg inmice for 14 days after 24 h of tumor inoculation. The effect of MEBL on the growth of transplantablemurine tumor, life span of EAC bearing mice, hematological profile and liver biochemical parameters(lipid peroxidation, antioxidant enzymes) were estimated. Treatment with MEBL decreased the tumorvolume and viable cell count thereby increasing the life span of EAC bearing mice and brought backthe hematological parameter more or less normal level. The effect of MEBL also decreases the levels oflipid peroxidation and increased the levels of glutathione (GSH), superoxide dismutase (SOD) andcatalase (CAT). The present work indicates that the methanol extract of Bryonia laciniosa exhibitedsignificant antitumor and antioxidant activity in vivo.