Plants have been used in traditional medicinal system for centuries. Bangladeshi medicinal plantshave received considerable attention from the researchers for evaluation of their bioactivity. As apart of our ongoing research of screening the Bangladeshi medicinal plants, the ethanolic extractof Dendrophthoe falcata have been chosen for the present study. The ethanolic extract of the leavesof the plant have been assessed for their antioxidant, antinociceptive, and general toxicity. Theextract showed potent antioxidant activity (IC50 5.1 µg/ml) using DPPH radical scavenging assay,which is comparable to the standard ascorbic acid (IC50 4.6 µg/ml). The extract significantly anddose dependently inhibited the acetic acid induced writhing in mice (71.2%, P < 0.001 and 28.0%,P < 0.05 for 500 and 250 mg/kg body weight, respectively). A general toxicity was assessed by asimple and low cost assay using brine shrimp lethality as an indicator. The extract showed lowlevel of toxicity (LC50 100 µg/ml). Using different chromatographic techniques, quercitrin (quercetin3-O-α-rhamnoside) was separated as the major component from the extract. The structure waselucidated by detailed 1D and 2D NMR and mass spectral analysis.