Ji-Jang-Soo (JJS) is known to have a detoxification effect. However, it is still unclear how JJS hasthese effects in experimental models. In this study, we investigated the effect of JJS on the viabilityof cells and production of cytokines in human T-cell line, MOLT-4 cells, and human mast cell line,HMC-1 cells. The MOLT-4 cells were cultured for 24 h in the presence or absence of JJS. As theresult, JJS (1/100 dilution) significantly increased the cell viability about 78% (P < 0.05) and alsoincreased the interleukin (IL)-2, and interferon (IFN)-γ production compared with media controlat 24 h. But had no effect on IL-4 production. Hypoxia mimic compound, desferroxamine (DFX)decreased the immune cell viability. Cell viability decreased by DFX was increased by JJS. Inconclusion, these data indicate that JJS may have an immune-enhancing effect.