The methanol extracts of the roots, root bark and stem of Berberis tinctoria, were investigated fortheir hepatoprotective activity against carbon tetrachloride (CCl4) induced toxicity in freshlyisolated rat hepatocytes, HEp-G2 cells and animal models. The methanol extracts were able tosignificantly normalise the levels of aspartate amino transferase, alanine aminotransferase, alkalinephosphatase, triglycerides, total proteins, albumin, total bilirubin and direct bilirubin, which werealtered due to CCl4 intoxication in freshly isolated rat hepatocytes and also in animal models. Theanti-hepatotoxic effect of the methanol extracts in vitro were observed at 600-1,000µg/mlconcentrations. A dose dependent increase in the percentage viability was observed when CCl4exposed HEp-G2 cells were treated with different concentrations of the methanol extracts. Thehighest percentage viability of HEp-G2 was observed at a concentration of 1,000 µg/ml. Theresults from the present investigations also indicate good correlation between the in vivo and invitro studies.