In this study, a new extraction method was developed and two kinds of flavonoids were extractedfrom the mature fruit of Vaccinium uliginosum L. of China. These two kinds of flavonoids wereanalyzed by spectral and identified by high-performance liquid chromatography (HPLC) andUV/Vis. The extract of the fruit was total acid hydrolyzed. TLC chromatography wassubsequently employed to identify the hydrolysate. Two kinds of aglycone flavonoids, quercetinand myricetin, were identified. At the same time PC chromatography was used to identify themonomer sugar in the flavonoids and it was verified as glucose. HPLC, UV/Vis, and Massspectrum analyses revealed that the flavonoids were quercetin 3-monoglucosides and myricetin3-monoglucosides.