The methanol extract and residual methanol fraction of Excoecaria agallocha L. (Euphorbiaceae)stem bark was investigated in this study by wheat rootlet and shoot growth inhibition, andantimicrobial bioassay. The methanol extract and residual methanol fraction showed highinhibitory effect on both the wheat rootlet (82-89%) and shoot growth (85-90%) compared tocontrol. The methanol extract showed a better and dose related inhibition on both the rootlet andshoot growth compared to residual methanol fraction. The IC50 value of methanol extract forrootlet and shoot were 2.88 µg/ml and 2.32 µg/ml, and of residual methanol fraction for rootletand shoot were 7.91 µg/ml and 4.45 µg/ml. The methanol extract and residual methanol fractiondid not show any antibacterial activity against the tested microorganisms of clinical isolatesEscherichia coli, Staphylococcus aureous, Pseudomonas aeruginosa, Proteus vulgaris and Bacillus subtilis.The plant has the potential to be a source of novel cytotoxic compound(s).