The crude ethanol extract of the stem bark of Trewia polycarpa (Family: Euphorbiaceae) wassubjected to acetic acid induced writhing inhibition, Brine Shrimp lethality bioassay and 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging assay for screening of analgesic, cytotoxic andantioxidant activity respectively. The extract produced significant (P < 0.001) writhing inhibition inacetic acid induced writhing in mice at the dose of 125, 250 and 500 mg/kg body weightrespectively, which were comparable to the standard drug diclofenac sodium. The extract showedsignificant lethality to Brine Shrimp and the LC50 value was 8 µg/ml. The extract showed prominentfree radical scavenging activity (IC50 about ~10 µg/ml) compare to standard drug ascorbic acid(IC50 about ~15 µg/ml). The results tend to suggest that the crude ethanol extract of the bark mightpossess analgesic, cytotoxic and antioxidant activities or active constituent(s) responsible for theactivities.