Callus cultures of Convolvulus microphyllus Sieb. was induced on Murashige and Skoog’s mediumsupplemented with 2,4-dichloro phenoxy acetic acid, 6-benzyl adenine, indole acetic acid andkinetin (1 ppm each). Methanolic extracts of whole plant, leaf, stem and leaf and stem calli weretested for anticonvulsant activity against standard drug phenytoin using maximal electroshockmodel on mice. It was observed that the animals treated with methanolic extracts of stem callus,leaf callus and whole plant (200 mg/kg, oral) showed significant protection against tonicconvulsions induced by transcorneal electroshock. Anticonvulsant activity of methanolic extractof stem callus was comparable to that of standard drug phenytoin.