Mucus is an aqueous gel complex with a constitution of about 95% water, high molecular weightglycoprotein (mucin), lipid, salts etc. Mucus appears to represent a significant barrier to theabsorption of some compounds. Natural mucoadhesive agent was isolated and purified from theaqueous extract of the seeds of prosopis pallida (PP). Formulated tablet with the isolated material bywet granulation method. Some natural edible substances are in consideration for candidates asmucoadhesive agents to claim more effective controlled drug delivery as an alternative to thecurrently used synthetic mucoadhesive polymers. Subjected the materials obtained from naturalsource i.e. PP and standard synthetic substance, sodium carboxymethyl cellulose for evaluation ofmucoadhesive property by various in vitro and in vivo methods. Through standard dissolution testand a model developed with rabbit, evaluated in vitro controlled release and bioadhesive property oftheophylline formulation. Mucoadhesive agent obtained from PP showed good mucoadhesivepotential in the demonstrated in vitro and in vivo models. The results suggest that the mucoadhesiveagent showed controlled release properties by their application, substantially. In order to assess thegastrointestinal transit time in vivo, a radio opaque X-ray study performed in healthy rabbit testing thesame controlled release formulation with and without bioadhesive polymer. Plasma levels oftheophylline determined by the HPLC method and those allowed correlations to the in vitromucoadhesive study results. Better correlation found between the results in different models. PP mayacts as a better natural mucoadhesive agent in the extended drug delivery system.