Radix Paeoniae rubra (PR) has been widely used for recovering acute inflammation with swellingand pain in Far East Asian countries. In order to investigate the anti-arthritic effect of PR herb-acupuncture, several behavioral parameters such as body weight, knee circumference, squeakingthreshold, and weight distribution ratio were investigated in an adjuvant-induced mono-arthritisrat model. We performed PR herb-acupuncture on Zusanli (ST36) of the right hind-leg once everysecond day for a total of 6 times from the second day of initiating arthritis with adjuvant injection,while the control group was treated with saline in the same way. In the experimental group, bodyweight significantly increased, knee circumference and squeaking threshold significantlydecreased indicating the reduction of edema, and both the pain and inflammatory swelling of thehind paws measured by incapacitance meter decreased. Conclusively, PR herb-acupuncture hasmore promising effect in alleviating arthritic pain and inflammation than saline-acupuncture inrat model, hence further clinical study is required.