This study reports the analgesic, anti-inflammatory and membrane-stabilizing property of alcoholicextract of Ophiorrhiza nicobarica (ON), a wild herb, used as an anti-infective ethnomedicine ofNicobarese and Shompen tribes of Great Nicobar Island, India. We for the first time investigated theanalgesic and antiinflammatory potential of this herb in acute, subacute and chronic model ofinflammation in Swiss albino mice and Wistar albino rats, along with sheep RBC-induced sensitivityand membrane stabilization. The acetic acid induced writhing, tail flick and tail immersion tests areused as a model for evaluating analgesic activity; while the carrageenin-induced paw oedema wasused as the model for acute inflammation, dextran-induced oedema as sub-acute and cotton-pellate-induced granuloma as chronic inflammatory model. The probable mode by which ON mediate itseffect on inflammatory conditions was studied on sheep RBC-induced sensitivity and membranestabilization. The in vitro results revealed that the ON extract possesses significant (P < 0.05) dosedependent analgesic and antiinflammatory activity at 200 and 300 mg/kg and its fractions at 50 mg/kg, p.o. respectively, compared to the control groups. However, the extract failed to exhibitmembrane-stabilizing property as it unable to reduce the level of haemolysis of RBC exposed tohypotonic solution. The acute toxicity studies of ON extract in rats and mice revealed that the extractwas nontoxic even up to 3.0 g/kg body weight of the animals, with a high safety profile. We haveisolated ursolic acid, β-sitosterol and harmaline respectively, from the bioactive part of the extract. Theresults indicated that the O. nicobarica is indeed beneficial in primary health care, and suggest that itsanti-inflammatory activity may not be related to membrane-stabilization.