We have developed the tablet formulation of Triticum aestivum (wheatgrass) and investigatedclinically, its effects in patients suffering from b-thalassemia (major) at K. T. Children Hospital,Civil Hospital, Rajkot. The tablets (wheatgrass powder 250 mg.) were given 3 times in a day for9 months. Blood samples were collected at the start, after 6 months and 9 months and analyzedfor various biochemical and hematological parameters. Treatment with wheatgrass formulationfor 9 months produced significant decrease in hemoglobin, total RBC, eosinophil and reticulocytecounts. The mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration(MCHC) were significantly increased was significantly increased. The serum ferritin was alsosignificantly decreased. There was no influence on serum magnesium, serum iron and Thiobarbituric acidreacting substances (TBARS). Our data indicate that treatment with wheatgrass on patients withß-thalassemia (major) may have beneficial effects in the form of a decrease in ineffectiveerythropoiesis, stimulation of hemoglobin synthesis in RBC, decrease in iron load and decrease ineosinophil count.