Gyejijakyakjimo-tang is a multi-herbal formula that is composed of nine medicinal herbs.Gyejijakyakjimo-tang has been reported to have antipyretic and analgesic effects. Gyejijakyakjimo-tanghas traditionally been used for goat and rheumatoid arthritis. However, analgesic and anti-inflammatory effects of Gyejijakyakjimo-tang has not been clarified yet. In this study, weinvestigated the analgesic and anti-inflammatory effect of the aqueous extract of Gyejijakyakjimo-tang. Weevaluated the aqueous extract of Gyejijakyakjimo-tang on Lipopolysaccharide (LPS)-inducedinflammation in murine raw 264.7 macrophage cells. For this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction(RT-PCR), prostaglandin E2 (PGE2) immunoassay, and nitric oxide (NO) detection wereperformed. Gyejijakyakjimo-tang suppressed PGE2 synthesis and NO production by inhibiting theLPS-induced expressions of COX-2 and iNOS mRNA in murine raw 264.7 macrophage cells.These results show that Gyejijakyakjimo-tang has the analgesic and anti-inflammatory effect bymostly suppressing COX-2 and iNOS expressions, and resulting in the inhibition of PGE2synthesis and NO production.