Recently a major goal in asthma therapy is to reduce or prevent the inflammatory response ofairway. Eosinophilic accumulation in the tissue is a prominent feature of allergic diseasesincluding asthma. Production of chemokines by bronchial epithelial cells may contribute to theallergic inflammation by recruiting eosinophils. In this study we evaluated the inhibitory effect ofGamichungsangbohatang (GMCSBHT), used traditionally in treating asthma, on secretion ofchemokines for eosinophils in human A549 epithelial cells. Chemokines such as eotaxin, RANTES, IL-8 were inhibited in a dose-dependent manner, but IL-16 showed no inhibition by GMCSBHT. Thesefindings indicate that GMCSBHT might be a therapeutic value in treating asthma by suppression ofchemokines secretion associated with local accumulation of eosinophils.