Methanol and aqueous extract of leaves of Trema orientalis Linn. were subjected to the potentialantioxidant and antibacterial activities. The pharmacological interest of this plant coupled withtraditional use (antidiarrhoeal, antiseptic, analgesic etc) prompted to test for antioxidant andantibacterial activities. The antioxidant potential of the methanolic extract was determined on thebasis of their scavenging activity of the stable 1,1-diphenyl-2-picryl hydrazyl free radical. IC50 ofthe methanol extract of T. orientalis was 110.25 µg/ml which indicated the strong antioxidantactivity of the plant. However the aqueous extract showed mild antioxidant activity. In case ofantibacterial activities test, the extract was subjected for its effectiveness against both Gram-positive and Gram-negative bacteria in agar diffusion method. The zones of inhibition producedby the crude methanol and aqueous extract against few sensitive strains were measured andcompared with those of standard antibiotic Gentamycin. It is evident that both extracts are activeagainst the bacteria at low concentrations. The obtained results provide a support for the use ofthis plant in traditional medicine and suggest its further advance investigation.