In the present investigation, we studied the effect of alcoholic extract of Moringa oleifera (M.oleifera) seed kernels on various experimental models of bronchial asthma. Significant (P < 0. 05)increase in preconvulsion time was observed due to pretreatment with M. oleifera when theguinea pigs were exposed to either acetylcholine (Ach) or histamine aerosol. This bronchodilatingeffect of M. oleifera was comparable to ketotifen fumarate. Spasmolytic effect of M. oleifera was alsoobserved by dose dependent inhibition of ideal contractions induced by Ach, 5HT, histamine andBaCl2. Alcoholic extract of M. oleifera produced significant dose dependent protection by eggalbumin and compound 48/80 induced mast cell degranulation. Pretreatment with alcoholicextract of M. oleifera also decreased carrageenan induced rat paw edema, which was comparableto that of standard diclofenac sodium. Minimum inhibitory concentration for alcoholic extract ofM. oleifera was low as compared to cold-water extract and hot water extract when antimicrobialactivity was tested against various respiratory pathogens like Escherichia coli (E. coli), Staphylococusaureus (S. aureus) and pseudomonas aeruginosa (P. aeruginosa). Our data suggest that antiasthmaticactivity of M. oleifera seed kernels may be due to its bronchodilator, anti-inflammatory, mast cellstabilization and antimicrobial activity.