The plant Eclipta prostrata, a member of the Compositae family, has folkloric reputation of beingused as a medicinal agent in Bangladesh. In the present investigation, attempt was taken toexplore the antimicrobial potency and cytotoxicity of its extractives and purified compounds. Themethanolic extract of the whole plant, its n-hexane, carbon tetrachloride, chloroform, aqueoussoluble fractions and two purified compounds, eclalbasaponin I (1) and II (2), obtained fromEclipta prostrata were subjected to screening for inhibition of microbial growth by the discdiffusion method at 300 and 100 µg/disc for extracts and pure compounds, respectively. In thiscase, the carbon tetrachloride and chloroform soluble fractions of the methanolic extract appearedvery potent in terms of both zone of inhibition and spectrum of activity. However, all theextractives were also subjected to brine shrimp lethality bioassay for preliminary cytotoxicityevaluation. Here, the carbon tetrachloride soluble fraction of methanolic extract revealed thestrongest cytotoxicity having LC50 of 1.318 µg/ml.