This study evaluated the anti-inflammatory potential of the crude alkaloidal fraction (CAF) ofmethanol extract of Solanum trilobatum Linn. (Solanacea) root in animal models of inflammation.Crude alkaloidal fraction at doses of 25, 50 and 100 mg/kg significantly (p < 0.01) reducedcarrageenan induced rat paw volume at 3 h after carrageenan challenge as compared to controlgroup of animals. CAF (25, 50 and 100 mg/kg) significantly (p < 0.01) and dose dependentlysuppressed cotton pellet induced granuloma formation. Topical application of CAF (1, 5 and 10mg/ear) markedly inhibited multiple application of TPA in mice. CAF elicited pronouncedinhibitory effects on formaldehyde and adjuvant induced arthritis in rats. These results indicatethat CAF of methanol extract of the Solanum trilobatum has anti-inflammatory activity in acute andchronic inflammation.