The objective of the present study was to assess the antipsychotic and antidiarrhoeal activities ofethanolic extract of roots of Rubia cordifolia in mice and rats. The antipsychotic activity of ethanolicextract of roots of Rubia cordifolia (ERC) was evaluated by observing its effect on amphetamine-induced stereotyped behavior in mice. Effect of ERC was also studied on motor coordination andlocomotion in mice. The antidiarrhoeal activity was evaluated using castor oil-induced diarrhoeaand excretion of sodium and potassium ions in the intestinal secretion in rats and gastrointestinaltransit in mice. The ERC inhibited amphetamine-induced stereotyped behaviour, diminishedlocomotion and impaired motor coordination. ERC inhibited castor oil-induced diarrhoea,decreased both sodium and potassium excretion in the intestine and decreased gastrointestinaltransit. Thus the present study confirms the anti-diarrhoeal activity of Rubia cordifolia. Furtherstudies are necessary to evaluate the potential of Rubia cordifolia as an antipsychotic.