The present study is aimed at finding the influence of silymarin (a flavonoid) (25 mg/kg & 50 mg/kg) instreptozotocin (STZ)-induced diabetic rats. Type 2 diabetes was induced by single intraperitonealinjection of STZ (100 mg/kg) to 3 days old rat pups. Silymarin was administered for 15 days afterthe animals were confirmed diabetic (75 days after STZ injection). Blood glucose, glycosylatedhemoglobin (HbA1c), lipid peroxides (LPO) levels and reduced glutathione (GSH) contents inpancreas and liver were estimated following the established procedures. Biochemical observations werefurther substantiated with histological examination of pancreas. Blood glucose and HbA1c levels, whichwere elevated by STZ, were lowered to physiological levels by the administration of silymarin. Thelevels of LPO were significantly increased in STZ-induced diabetic rats. Silymarin reduced the LPOlevels in both pancreas and liver. GSH contents which were reduced significantly in pancreas andliver of STZ-induced diabetic rats were brought back to near normal levels by silymarin treatment.Multifocal necrotic and degenerative changes of pancreas in STZ-diabetic rats were minimized to nearnormal morphology by administration of silymarin as evident by histopathological examination.Silymarin showed a dose dependent protective effect on STZ-induced β-cell damage. It could beattributed to the antioxidative and free radicals scavenging properties of the flavonoid. Thus, itmay be considered as a natural antioxidant with potential therapeutic application in the treatmentof type 2 diabetes.