The hepatoprotective activity of the alcoholic extract of Rosa damascena was studied againstparacetamol induced acute hepatotoxicity in rats. Liver damage was assessed by estimatingserum enzyme activities of aspartate aminotransferase, alanine aminotransferase, alkalinephosphatase and histopathology of liver tissue. Pre- and post-treatment with ethanolic extractsshowed a dose-dependent reduction of paracetamol induced elevated serum levels of enzymeactivity. The mechanism underlying the protective effects was assayed in vitro and the R.damascena extracts displayed dosedependent free radical activity using DPPH (IC50 = 162.525 μg/ml)and TBA method. The hepatoprotective action was confirmed by histopathological observation.The ethanolic extracts reversed paracetamol induced liver injury. These results suggest that thehepatoprotective effects of R. damascena extracts are related to its antioxidative activity.