Trema orientalis Linn. is commonly grown in many parts of Bangladesh. Its leaves have been used foranalgesic and anti-diarrhoeal activity in traditional medicine. This study evaluates the potentialanalgesic and anti-diarrhoeal activity of methanol and aqueous extracts of leaves in experimental aceticacid induced writhing and castor oil induced diarrhoea in mice. The aqueous extract of leaves showedsignificant (P < 0.001) analgesic effect in acetic acid induced writhing in mice at a dose of 500 mg/kgbody weight In castor oil induced antidiarrhoeal screening both extract increased latent period (P <0.025) and decrease the number of stool (P < 0.025) at the dose of 500 mg/kg body weight comparablewith that of the standard drug loperamide. The results provide a support for the use of this plant intraditional medicine and suggest its further investigation.