In this present study Indian traditional Ayurvedic herbal formulation Amritaristo has been studiedto assess the general pharmacological effect on mice. The drug showed no significant activity on theneuropharmacological test models experimented. The increased pentobarbital sleeping time wasconsidered related with hepatic metabolism of pentobarbital. The formulation exhibited a non-significant reduction of gastrointestinal motility, and devoid of any acute diuretic activity. Thetested drug revealed antidiarrhoeal activity on castor oil-induced model, whereas on magnesiumsulphate-induced model no effect was observed.