The MeOH extract of seeds of Cerbera odollam Gaertn. (Apocynaceae) was screened for its antibacterial,cytotoxic and neuropharmacological activities. The extract showed moderate anti-bacterialactivity against Salmonella typhi, Streptococcus saprophyticus, and Streptococcus pyogenes. It exhibitedhigh level cytotoxicity against brine shrimp (LC50: 3 µg/ml). The extract potentiated pentobarbitalinduced sleeping time in mice which was further supported by the exploratory behavior test atdose of 25 mg/kg. The overall results tend to suggest the antibacterial, cytotoxic and CNSdepressant activities of the extract.