Allergic conjunctivitis is the most common allergic disease. These diseases are severe & frequentwhich requires search of new treatments. The aim of the study was to investigate the effects ofTinospora cordifolia (TC), Rubia cordifolia Linn. (RC) on experimentally induced allergic conjunctivitis in rats.In this study, dried water soluble extracts of TC and RC. (250 and 500 mg/kg, p.o. for 7 days)were evaluated for their antiallergic activity in Wistar rats. They were tested for inhibition of eggalbumin-induced vascular permeability, inhibition of histamine release from the rat conjunctivaas well as in histamine content in tears. TC and RC showed significant (P < 0.05) inhibition invascular permeability, inhibition in histamine release from the rat conjunctiva which is reflectedby reduced level of histamine content in tears. The activities were found to be comparable toazelastine hydrochloride. These results suggest that the inhibitory effect on egg albumin-inducedexperimental allergic conjunctivitis in rat may be due to the antihistaminic activity of TC and RC.Our studies provide evidence that TC and RC may be beneficial in the treatment of allergicconjunctivitis.