Folic acid inhibits necrosis and apoptosis in ischemic and reperfusioninduced injury in rat liver > Volume 09 - 2009

본문 바로가기

사이트 내 전체검색


 » New Articles(2011~Present)
 » Articles (2001~2010)
      ∙ Volume 10 - 2010
      ∙ Volume 09 - 2009
      ∙ Volume 08 - 2008
      ∙ Volume 07 - 2007
      ∙ Volume 06 - 2006
      ∙ Volume 05 - 2005
      ∙ Volume 04 - 2004
      ∙ Volume 03 - 2003
      ∙ Volume 02 - 2002
      ∙ Volume 01 - 2001
 

Oriental Pharmacy and Experimental Medicine
Volume 09 - 2009
Date: February 04, 2008

Journal: Pages 67-73

March 2009 | Folic acid inhibits necrosis and apoptosis in ischemic and reperfusioninduced injury in rat liver…

Pronobesh Chattopadhyay1,2,*, Gunjan Shukla3 and Arun Kumar Wahi1

첨부파일

Summary

​Temporary clamping of the portal triad is a common strategy to minimize bleeding during livertransplantation. Increasing evidences suggests that oxygen derived free radicals and reintroduction ofoxygen in ischemic tissue lead to ischemic and reperfusion injury (I/R) and lead to apoptosis andnecrosis. Adult Wistar rat subjected to 60 min of partial liver ischemia followed by three hourreperfusion. Eighteen Wister rats were divided into sham-operated control group (I) (n = 6),ischemia and reperfusion group (II) (n = 6), folic acid treated group (1 mg/kg body weight/dailyby oral route for 7 days before induced ischemia reperfusion maneuver) (III) (n = 6). Apoptoticand necrotic hepatocytes, mitochondrial antioxidant enzymes were measured. Liver injury wasassessed by alanine transaminases (ALT), aspartate transaminases (AST), liver histopathologyand electron microscopy. An ischemic and reperfusion hepatocellular injury was indicated byincreased serum-ALT, AST, histopathology and electron microscopy studies. Apoptotic andnecrotic cells were increased which was revealed by flow cytometry in I/R group. Pre- treatmentwith folic acid significantly decreased serum -ALT, AST levels, apoptotic and necrotic cells after 1h ischemia followed by 3 h of reperfusion. Histopathology and TEM studies showed markedlydiminished hepatocellular injury in folic acid pretreated rats during the hepatic I/R, whichreached a level comparable to saline-treated rat of sham operated group. On the basis of ourfindings it may be concluded that folic acid afforded significant protection from necrosis andapoptosis in I/R injury.


This website Copyright (c) 2014 Institute of Korean Medicine Kyung Hee University All rights reserved.
Top View
Mobile View