Albizia julibrissin (AJ) has been used widely as a traditional medicine. In macrophages nitric oxide(NO) is released as an inflammatory mediator and has been proposed to be an importantmodulator of many pathophysiological conditions including inflammation and carcinogenesis. Inthis study we have examined the NO inhibition effect of 85% methanol extracts of AJ in mousemacrophage. Lipopolysaccharide (LPS) has been reported to induce production of NO. Extracts ofAJ (1, 10, 100 μg/ml) suppressed nitric oxide production in LPS-stimulated (100 μg/ml) mouse(C57BL/6) macrophages and analyzed by ELISA. In addition, it also attenuated the expression ofinflammatory products like Interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), and inducible NOS(iNOS) as assessed by immunoblotting with specific antibodies. These results suggest that 85%methanol extracts of AJ would be useful in inflammatory diseases.