Pterocarpus marsupium, Clitoria ternatea, and Sanseveiria cylindrica are some of the important andendangered medicinal plant species of India. Despite of medicinal properties, antibacterialpotential of the plants have not yet been explored. The present study was designed to optimizethe in vitro technique for micropropagation and to screen the extracts from leaves and in vitroraised calli for antibacterial properties. Excised leaf-explants from the parent plants were surfacesterilized and cultivated on Murashige & Skoog’s (MS) medium containing N6-benzyladenine(BA) in concentrations of 1, 2, 5, and 10 µM. Optimal growth of calli was noticed at aconcentration of 5 µM, therefore the extracts from calli grown at this concentration were furtherstudied for antibacterial activity. Both alcoholic and aqueous extracts from leaves of respectiveplants, and their in vitro raised calli were tested for antibacterial activity by agar well diffusionmethod against a range of Gram-positive and Gram-negative bacteria. Aqueous extracts showedantibacterial activity against limited number of bacterial species; notably the extracts of C. ternateawhich showed antibacterial activity against Streptococcus pyogenes, Bacillus subtilis and Bacilluscereus. Alcoholic extracts of all three plants showed antibacterial activity against a wider range ofbacteria. Among the Gram-positive bacteria, extracts from C. ternatea showed strong antibacterialactivity against Bacillus spp., whereas the extracts of S. cylindrica showed good antibacterialpotential for Staphylococcus aureus, S. epidermidis and S. pyogenes. The extracts from all three plantsshowed antibacterial activity against Gram-negative bacteria, including, Salmonella spp. andShigella dysenteriae; organisms causing enteric fever and dysentery. In most of the cases, theextracts from respective calli showed comparable, and in some cases better, result in comparisonto the extracts from parent leaves. To the best of our knowledge this is the first preliminary reporton antibacterial potential, especially through calli extracts, of these plants; and in vitro cultivationof the explants may be used to obtain phytotherapeutic compounds.