Antibacterial potential of the extracts derived from leaves and in vitro raisedcalli of medicinal plants Pterocarpus marsupium Roxb., Clitoria ternatea L.,and Sanseveiria cylindrica Bojer ex Hook > Volume 09 - 2009

본문 바로가기

사이트 내 전체검색


 » New Articles(2011~Present)
 » Articles (2001~2010)
      ∙ Volume 10 - 2010
      ∙ Volume 09 - 2009
      ∙ Volume 08 - 2008
      ∙ Volume 07 - 2007
      ∙ Volume 06 - 2006
      ∙ Volume 05 - 2005
      ∙ Volume 04 - 2004
      ∙ Volume 03 - 2003
      ∙ Volume 02 - 2002
      ∙ Volume 01 - 2001
 

Oriental Pharmacy and Experimental Medicine
Volume 09 - 2009
Date: June 18, 2007

Journal: Pages 174-181

June 2009 | Antibacterial potential of the extracts derived from leaves and in vitro raisedcalli of medicinal plants Pterocarpus marsupium Roxb., Clitoria ternatea L.,and Sanseveiria cylindrica Bojer ex Hook…

M Shahid1,*, A Shahzad2 and M Anis2

첨부파일

Summary

​Pterocarpus marsupium, Clitoria ternatea, and Sanseveiria cylindrica are some of the important andendangered medicinal plant species of India. Despite of medicinal properties, antibacterialpotential of the plants have not yet been explored. The present study was designed to optimizethe in vitro technique for micropropagation and to screen the extracts from leaves and in vitroraised calli for antibacterial properties. Excised leaf-explants from the parent plants were surfacesterilized and cultivated on Murashige & Skoog’s (MS) medium containing N6-benzyladenine(BA) in concentrations of 1, 2, 5, and 10 µM. Optimal growth of calli was noticed at aconcentration of 5 µM, therefore the extracts from calli grown at this concentration were furtherstudied for antibacterial activity. Both alcoholic and aqueous extracts from leaves of respectiveplants, and their in vitro raised calli were tested for antibacterial activity by agar well diffusionmethod against a range of Gram-positive and Gram-negative bacteria. Aqueous extracts showedantibacterial activity against limited number of bacterial species; notably the extracts of C. ternateawhich showed antibacterial activity against Streptococcus pyogenes, Bacillus subtilis and Bacilluscereus. Alcoholic extracts of all three plants showed antibacterial activity against a wider range ofbacteria. Among the Gram-positive bacteria, extracts from C. ternatea showed strong antibacterialactivity against Bacillus spp., whereas the extracts of S. cylindrica showed good antibacterialpotential for Staphylococcus aureus, S. epidermidis and S. pyogenes. The extracts from all three plantsshowed antibacterial activity against Gram-negative bacteria, including, Salmonella spp. andShigella dysenteriae; organisms causing enteric fever and dysentery. In most of the cases, theextracts from respective calli showed comparable, and in some cases better, result in comparisonto the extracts from parent leaves. To the best of our knowledge this is the first preliminary reporton antibacterial potential, especially through calli extracts, of these plants; and in vitro cultivationof the explants may be used to obtain phytotherapeutic compounds.


This website Copyright (c) 2014 Institute of Korean Medicine Kyung Hee University All rights reserved.
Top View
Mobile View