Aldose reductase (AR) has been reported to play an important role in sugar-induced cataract. Inthe present study, the AR inhibitory activity of Enicostemma hyssopifolium (EH), Gymnema sylvestre,Eclipta alba, and Tinospora cordifolia (TC) were studied along with their effect on sugar-inducedcataractogenic changes in sheep lenses in vitro. AR inhibitory activity of the aqueous extracts ofplants and their anticataract potentials were evaluated in vitro in sheep lenses, considering theactivity of normal sheep lenses as 100%. The concentration of the plant extract that showed maximumactivity was selected to further study its effect on galactose-induced polyol accumulation in vitro.The IC50 values of EH and TC were calculated to be 102 and 85 µg/ml, respectively. EH showed asignificant inhibition (61.3%) in polyol accumulation followed by TC (53.1%). EH and TCpossesses a significant anticataract activity in vitro and its anticataract potential could be relatedwith its AR inhibitory effect.