Curcumin (CMN) is known to have beneficial role in anorexia, coryza, cough, diabetic wounds,and hepatic disorders apart from its inherent antioxidant effects. Therefore, the present study wasaimed to evaluate antioxidant effect of CMN in prevention of nephrolithiasis in rats-induced byethylene glycol (EG) and Vitamin D3 (Vit. D3). Male Wistar rats (175 - 200 g) were randomized ingroups like control, EG + Vit. D3 induced nephrolithiatiatic rats, CMN treated rats, CMN + EG +Vit. D3 treated rats, Vit. E + EG + Vit. D3 treated rats. Urine was collected weekly throughout theexperimental protocol and estimated for calcium oxalate (CaO) count. After completion ofexperimental protocol serum was estimated for blood urea nitrogen and creatinine. Both thekidneys were excised and used to evaluate levels of biomarkers of oxidative stress and calciumoxalate crystal deposition by histopathological studies. Administration of EG and Vit. D3 to ratsresulted in increased oxidative stress, hyperoxaluria and renal deposition of CaO crystals.Supplementation with CMN improves kidney function, reduces elevated oxidative stress, urinaryoxalate level and renal deposition of CaO which shows its protective action in nephrolithiasis.The increased deposition of stone in the kidney and stone forming constituents of nephrolithiaticrats were effectively lowered by treatment of CMN.