The present study was undertaken to evaluate the protective effect of ethanol (EtAS), ethyl acetate(EAAS) and aqueous (AQAS) extracts of Argyreia speciosa (AS) roots against Isoproterenol (ISO)-induced myocardial infarction in rats. The animals were exposed to isoproterenol (200 mg/kg.s.c) twice at an interval of 24 hrs. Cardioprotective effect was assessed by observing ECGparameters, serum marker enzymes and histopathology of the heart. Pretreatment of EAAS, andEtAS (200 mg/kg) resulted in a significant (P < 0.001) increase in P wave, QRS complex and R-Rinterval, whereas heart rate, QT interval and cardiac cycle were maintained near to normal values.EtAS and EAAS showed significant (P < 0.05; P < 0.001) reduction in all the tested diagnostic markerscompared to ISO treated group. Histological studies on the structural changes of heart tissuesupported the protective activity of AS. The result suggest that treatment of AS prior to ISO has asignificant role in protecting the animals from ISO induced myocardial infarction.