Typha orientalis’ stem (TOS) is traditionally used as an herbal medicine for difficulty in urination,galactophoritis purulenta, whooping cough, and allergic dermatitis. However, its effect in experimentalmodels remains unknown. Here, we report the effect of TOS on the phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-induced inflammatory cytokine production andextracellular signal-regulated kinase (ERK) activation in the human mast cell line, HMC-1. TOSinhibited PMA plus A23187-induced cytokines such as tumor necrosis factor-alpha (TNF-α) andinterleukin (IL)-6. Maximal inhibition rate of TNF-α and IL-6 production by TOS (1 mg/ml) wasabout 44.02%, and 45.20%, respectively (P < 0.05). In addition, TOS inhibited the expression ofTNF-α and IL-6 mRNA under the same condition. Moreover, TOS partially blocked PMA plusA23187-induced ERK phosphorylation. These results suggested TOS could inhibit the cytokineproduction through blocking of ERK activity.