An examination of the kinetic properties of UDP-glucose, (1,3)-β-glucans (callose) synthase, frommung bean seedlings (Sorbus commixta cortex) shows that these enzymes have a complex relationship withUDP-glucose and various effectors. Fluorescence assay showed that deoxynojirimycin increasedthe inhibitory effect of (1,3)-β-glucan synthase in a concentration-dependent manner. Theinhibitory effect of sakuranetin (34.34%) was higher than that of deoxynojirimycin (80.63%). Diskdiffusion method revealed that sakuranetin inhibited the growth of Candida albicans to a 1.5 mminhibition zone. These results suggest that sakuranetin, isolated from Sorbus commixta cortexextract, can be used as stable antifungal material.