Vitex rotundifolia (V. rotundifolia) has been used for treating headache, dizziness, toothache andremoval of fever as a traditional medicine in Korea. In the present study, we examined theantioxidant and anti-inflammatory activities of 85% methanol extract of V. rotundifolia. In variousradical scavenging assays, V. rotundifolia exhibited strong scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl free radical, superoxide radical, nitric oxide. To elucidate the anti-inflammatoryproperties of V. rotundifolia, we investigated the inhibition effects of nitric oxide production inIFN-gamma and LPS-stimulated mouse peritoneal macrophages. V. rotundifolia suppressed nitricoxide production, iNOS and COX-2 expression dose-dependently through suppression of NF-êBactivation without notable cytotoxicity. These findings mean that V. rotundifolia may be beneficialin oxidative stress-mediated inflammatory disorders.