Acute (24 h) and chronic (90 days) oral toxicity studies on the ethanol extract of Trigonella foenum-graecum Leguminosae (L.) seeds were carried out. Acute dosages were 0.5, 1.0 and 3 g/kg whilechronic dosage was 100 mg/kg per day of the extract. All morphological, biochemical, haematologicaland spermatogenic changes, in addition to mortality, body weight changes and any change invital organs were recorded. Histopathological investigations were done on vital organs. Growtharrest in the treated animals was observed. The treated mice gained no significant weight duringchronic treatment while there was a significant gain in body weight of the control group mice.Biochemical studies revealed a significant decrease in blood sugar levels of fenugreek treatmentgroups while haematological parameters remained comparable to the control. In the treatment,male group there was a significant decrease in weight of testes as compared to the control. Therewas a marginal weight gain in kidney weight of mice after chronic treatment as compared to thecontrol. Fenugreek chronic treatment caused a highly significant spermatotoxic effects in male mice.