The flowers of Nyctanthes arbor-tristis Linn. of Oleaceae widely used in Ayurvedic system ofmedicine for the treatment of diuresis, liver disorder, spleen enlargement sciatica, bitter,stomachic, carminative and tonic to hair. The aim of the present study was to evaluate thealcoholic and aqueous extracts of the flowers of Nyctanthes arbor-tristis for hepatoprotective effectagainst carbontetrachloride induced liver damage in rats. Administration of alcoholic andaqueous extracts of the leaves of Nyctanthes arbor-tristis protect the liver from toxic effects ofcarbontetrachloride by reducing the elevated levels of Serum glutamate pyruvate transaminase,Serum glutamate oxaloacetate transaminase, Alkaline phosphatase and serum bilirubin. Resultsrevealed that both the alcoholic and aqueous extracts showed significant hepatoprotectiveactivity by reducing the elevated levels of biochemical parameters at a dose of 200 mg/kg bodyweight. The results were supported by histopathological studies of liver samples which showedregeneration of hepatocytes by the extracts.