The entire plant of Leptadenia reticulata (Asclepidaceae) is extensively used as lactogen, traditionally, inveterinary practice. The plants of Dregea volubilis and Pentatropis microphylla (Asclepidaceae) arenow used as its substitute and sometimes replace the original drug as traditional lactogen. Thelactogenic potential of these drugs was studied in rats using, pup weight, weight of mother,parenchyma percentage, secretary rating, estimation of total protein content and glycogen contentof mammary glands tissues as assessment parameters. HPTLC profiles of bioactive extracts werealso generated to serve the authentification needs. The results of present studies show thatP.microphylla forms a better substitute over D. volubilis.