Verbena officinalis (Verbenaceae) has been used as herbal medicine or health supplement in bothWestern and Eastern countries for centuries. In the present study, we examined the anti-inflammatory and antioxidant activities of the methylene chloride fraction of V. officinalis (VMC).To elucidate the anti-inflammatory properties of VMC, we investigated the inhibition effects ofnitric oxide production in interferon-gamma (IFN-γ) and lipopolysaccharide-stimulated mouseperitoneal macrophages. VMC suppressed nitric oxide production, inducible nitric oxide synthase andcyclooxygenase-2 expression dose-dependently without notable cytotoxicity. In various radicalscavenging assays, VMC exhibited strong scavenging effect on DPPH radical, superoxide radical,nitric oxide radical and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical. VMC alsoshowed potent reducing power. These findings strongly suggest that VMC may be beneficial inoxidative stress-mediated inflammatory disorders.