Lycii fructus is the fruit of Lycium chinense Miller and is part of the Solanaceae family. Lycii fructusproduces various effects such as hypotensive, hypoglycemic, anti-pyretic, and anti-stress activities. Lyciifructus is known to contain betaine, carotene, nicotinic acid, zeaxanthin, and cerebroside. In thepresent study, the effects of Lycii fructus aqueous extract on lipopolysaccharide (LPS)-inducedinflammation in murine raw 264.7 macrophage cells were investigated. In this study we utilizedthe 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and nitric oxide (NO) detection. Lycii fructusaqueous extract suppressed NO production by inhibiting the LPS-induced expressions ofinducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-α) mRNA and iNOSprotein in murine raw 264.7 macrophage cells. Also, Lycii fructus aqueous extract suppressed theactivation of nuclear factor-kappa B (NF-κB) in the nucleus. These results demonstrated that Lyciifructus aqueous extract causes an anti-inflammatory effect that was likely produced by thesuppression of iNOS expression through the down-regulation of NF-êB binding activity.