Aqueous extract of Lycii fructus suppresses inflammation through theinhibition of nuclear factor kappa B signal pathway in murine raw 264.7macrophages > Volume 10 - 2010

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Oriental Pharmacy and Experimental Medicine
Volume 10 - 2010
Date: August 24, 2010

Journal: Pages 155-164

September 2010 | Aqueous extract of Lycii fructus suppresses inflammation through theinhibition of nuclear factor kappa B signal pathway in murine raw 264.7macrophages…

Beum-Seuk Kim1, Hyung-Ho Lim1, Yun-Kyung Song1, Yun-Hee Sung2, Sung-Eun Kim2, Hyun-Kyung Chang2, Mal-Soon Shin2, Chang-Ju Kim2, Hyejung Lee3 and Dong-Hee Kim4,*

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Summary

​Lycii fructus is the fruit of Lycium chinense Miller and is part of the Solanaceae family. Lycii fructusproduces various effects such as hypotensive, hypoglycemic, anti-pyretic, and anti-stress activities. Lyciifructus is known to contain betaine, carotene, nicotinic acid, zeaxanthin, and cerebroside. In thepresent study, the effects of Lycii fructus aqueous extract on lipopolysaccharide (LPS)-inducedinflammation in murine raw 264.7 macrophage cells were investigated. In this study we utilizedthe 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and nitric oxide (NO) detection. Lycii fructusaqueous extract suppressed NO production by inhibiting the LPS-induced expressions ofinducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-α) mRNA and iNOSprotein in murine raw 264.7 macrophage cells. Also, Lycii fructus aqueous extract suppressed theactivation of nuclear factor-kappa B (NF-κB) in the nucleus. These results demonstrated that Lyciifructus aqueous extract causes an anti-inflammatory effect that was likely produced by thesuppression of iNOS expression through the down-regulation of NF-êB binding activity.


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